Automated, parallel mass spectrometry imaging and structural identification of lipids
Shane R Ellis, Martin R. L. Paine, Gert B. Eijkel, Josch K. Pauling, Peter Husen, Mark W. Jervelund, Martin Hermansson, Christer S. Ejsing, Ron M. A. Heeren
Nature Methods
We report a method that enables automated data-dependent acquisition of lipidtandem mass spectrometry data in parallel with a high-resolution massspectrometry imaging experiment. The method does not increase the total imageacquisition time and is combined with automatic structural assignments. Thislipidome-per-pixel approach automatically identified and validated 104 unique molecular lipids and their spatial locations from rat cerebellar tissue.