DNA-damage happens in living cells as often as 10,000 times per human cell a day due to numerous reasons. Some of the lesions occur as a consequence of exposure to various kinds of radiation, and can be expressed as as single or double strand breaks, as well as formation of a pyrimidine dimer, base loss, base change or cross-linkage.
To prevent cell death (apoptosis), the DNA damage is sought mended through various DNA repair pathways. Here two of the most important ones are expanded upon: Non-homologous repair of double strand breaks and UV-damage repair by photolyase.
Non-homologous repair of double strand breaks
The DNA repair process is quite complicated and involves multiple steps that all features different length scales as well as time scales. The first repair protein to arrive to the DNA nick is the MRN complex, consisting of the three proteins MRE11, RAD50 and NBS11, which initiates the non-homologous repair process for double strand breaks. The process is roughly described in Figure 1.
Naturally, the recruitment time of the MRE11, RAD50 and NBS11 is important for the time scale of the repair process, and to calculate the recruitment time, a mean of transportation of the repair proteins need to be decided. Here the passive transport method of diffusion is chosen to study the recruitment.
As diffusion can be described as a random walk, this allows for the construction of both a computational model and an analytical model of the recruitment time. For the computational approach a model with spherical symmetry is constructed with measurements as a human bone marrow cell, as seen in Figure 2.
For the analytical model, a property of the system called the first passage time is calculated. The first passage time is the time it takes for a diffusing protein to hit a target for the very first time. This is completely analogous to MRE11, RAD50 and NBS1 hitting the nucleus.
To find the first passage time, the starting point is the diffusion equation, which is solved for fast diffusion (t → 0) and slow diffusion (t → ∞).
The results of both the analytical model and the computations can be found in Figure 3.
The results show that diffusion can be a plausible theory for the transportation of MRE11 and NBS1 into the nucleus. Further investigations can be made to expand the knowledge of the different steps found in Figure 1.
UV radiation is not a powerful as ionising radiation, and it will not make single or double strand breaks in the backbone of the DNA. Instead UV radiation can lead to the formation of a photoproduct between two adjacent pyrimidine rings in the DNA, which can lead to mutagenesis and cancer if not repaired. To avoid this, a specialised enzyme called as DNA photolyase could interact with the photo lesion and repair it. Important for the repair to happen is the binding of the DNA and the coenzyme Flavin adenine dinucleotide (FAD). Photolyase is structurally highly homologous to a protein called cryptochrome and both proteins are biological activated similar, namely through Flavin co-factor photoexcitation. However cryptochrome cannot repair UV-damaged DNA. We use MD to investigate the binding between photolyase and the UV-damaged DNA and between cryptochrome and the UV-damaged DNA to get insight into important factors that govern the binding of UV-damaged DNA and reveal why cryptochrome cannot have this functionality.